منابع مشابه
Labeling nuclear DNA with hoechst 33342.
A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells. One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This pro...
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DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are des...
متن کاملDAPI-fluorescent fading: a problem in microscopy or a way to measure nuclear DNA content?
In observation by confocal or conventional fluorescence microscopy, the retardation of the lost in fluorescence, from highest signal of fluorescence to lowest intensity are important factors in order to obtain accurate images. This problem is very common in fluorochromes for nuclear DNA and especially for DAPI stain. The fluorescence of DAPI is rapidly lost when it is exposure to excitation by ...
متن کاملFlow Cytometric DNA-Analysis of Plant Protoplasts Stained with DAPI
Ingunn Ulrich and Wolfgang Ulrich Institut für Genetik. Universität Hohenheim , Garbenstraße 30, D-7000 Stuttgart-Hohenheim , Bundesrepublik Deutschland Z. Naturforsch. 41c, 1052 — 1056 (1986); received July 23, 1986 Flow Cytometry, Plant Protoplasts, Protoplasts Preparation, D N A , D A PI Protoplasts prepared from leaves of various higher plants were stained with the specific DNA fluorochrome...
متن کاملPolarized fluorescence correlation spectroscopy of DNA-DAPI complexes.
We discuss the use of fluorescence correlation spectroscopy for the measurement of relatively slow rotations of large macromolecules in solution or attached to other macromolecular structures. We present simulations and experimental results to illustrate the range of rotational correlation times and diffusion times that the technique can analyze. In particular, we examine various methods to ana...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2011
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5556